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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_HSC3
Organism:Homo sapiens
UniGene Library ID:1662
Tissue Description:Stem cell, purified CD34-positive T-negative peripheral blood stem cells from a patient in the chronic phase of chronic myelogenous leukemia (CML)
Library Keywords:bone marrow, non-normalized, chronic myelogenous leukemia, flow-sorted, hematopoietic cell, CGAP, EST, Krizman protocol 2, unknown developmental stage, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1152
#Sequences Generated to Date:712

Library Preparation Details

Description:mRNA made from lymphoid tissue, cDNA made by oligo-dT priming. Directionally cloned. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383.
R. Site1:
R. Site2:
Lab Host:DH10B
Vector:pAMP1
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:Herbert Morse, M.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Krizman Laboratory