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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_HN5
Organism:Homo sapiens
UniGene Library ID:1705
Tissue Description:Gingival keratinocytes, primary cell line.
Library Keywords:normal, non-normalized, bulk, CGAP, EST, Stratagene non-normalized, unknown developmental stage, size fractionated, directionally cloned, phagemid, oligo-dT primed, gum

Clones and Sequences

#Clones Generated to Date:384
#Sequences Generated to Date:327

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. 5' adaptor sequence: 5' AATTCGGCACGAG 3' 3' GCCGTGCTC 5' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3' EcoRI site appears to have been lost in a fraction of the clones. Library constructed by Stratagene; available through Mary May, PhD (Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, NIH; mmay@yoda.nidr.nih.gov).
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:SOLR (kanamycin resistant)
Vector:Bluescript SK-
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Chong Heon Lee, D.D.S., Mary May, J. Silvio Gutkind, Ph.D., Myung Hee Park, PhD.
Library Producer:Stratagene, Inc.