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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Co16
Organism:Homo sapiens
UniGene Library ID:1540
Tissue Description:Colon cancer
Library Keywords:colon, bulk, subtracted, CGAP, EST, Soares subtracted, unknown developmental stage, phagemid, colorectal cancer

Clones and Sequences

#Clones Generated to Date:18432
#Sequences Generated to Date:10161

Library Preparation Details

Description:Plasmid DNA from the normalized library NCI_CGAP_Co10 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1057416-1061255, and 1144584-1145351). Subtraction by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier: Ilan Kirsch, M.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory