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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Kid12
Organism:Homo sapiens
UniGene Library ID:1536
Tissue Description:Kidney, 2 pooled cancers, clear cell type
Library Keywords:kidney, bulk, adult, subtracted, CGAP, EST, Soares subtracted, phagemid, oligo-dT primed, clear cell renal carcinoma

Clones and Sequences

#Clones Generated to Date:17664
#Sequences Generated to Date:9670

Library Preparation Details

Description:Plasmid DNA from the normalized library NCI_CGAP_Kid5 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1323912-1325831, 1471368-1472903 and 1492104-1493255). Subtraction by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Christopher A. Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory