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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Stanley Frontal SN pool 1
Organism:Homo sapiens
UniGene Library ID:1480
Tissue Description:
Library Keywords:normal, frontal cortex, bulk, adult, subtracted, EST, size fractionated, plasmid vector, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:65

Library Preparation Details

Description:Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male schizophrenics, ages 30 and 32 (S-30, S-121) subtracted by pool of two mentally normal individuals, male, age 48 and female, age 35 (S-65, S-136). Tissues were obtained from the Stanley Neuropathology Consortium ( Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918,].
R. Site1:EcoRI
R. Site2:
Lab Host:DH10B (phage-resistant)
Vector:pCR2.1-TOPO (Invitrogen)
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: