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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Stanley Frontal SN individual
Organism:Homo sapiens
UniGene Library ID:1479
Tissue Description:
Library Keywords:frontal cortex, bulk, adult, subtracted, EST, male, size fractionated, plasmid vector, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:68

Library Preparation Details

Description:Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. 34 yo schizophrenic male (S-11) subtracted by 28 yo mentally normal male (S-37). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
R. Site1:EcoRI
R. Site2:XhoI
Lab Host:DH10B (phage-resistant)
Vector:pCR2.1 (Invitrogen)
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: