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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Pr28
Organism:Homo sapiens
UniGene Library ID:1410
Tissue Description:Prostate, normal tissue
Library Keywords:prostate, normal, bulk, adult, subtracted, CGAP, EST, Soares subtracted, male, phagemid

Clones and Sequences

#Clones Generated to Date:48384
#Sequences Generated to Date:29184

Library Preparation Details

Description:Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo.
R. Site1:
R. Site2:
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Michael J. Brownstein, M.D., Ph.D., Michael Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory