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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Br22
Organism:Homo sapiens
UniGene Library ID:7210
Tissue Description:Breast, invasive ductal carcinoma, three pooled samples
Library Keywords:mammary gland, invasive, non-normalized, bulk, adult, CGAP, EST, LTI non-normalized, female, size fractionated, directionally cloned, phagemid, oligo-dT primed, ductal carcinoma of the breast

Clones and Sequences

#Clones Generated to Date:2304
#Sequences Generated to Date:481

Library Preparation Details

Description:Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5 kb. Constructed by Life Technologies.
R. Site1:NotI
R. Site2:SalI
Lab Host:DH10B (T1-phage resistant)
Vector:pCMV-SPORT6
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Chris Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Life Technologies, Inc.