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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Barstead aorta HPLRB3
Organism:Homo sapiens
UniGene Library ID:1332
Tissue Description:
Library Keywords:normal, non-normalized, bulk, adult, EST, male, aorta, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:199

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCGGATCCAAC 3' and 5' GTTGGATCCG 3'], digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed by Bob Barstead.
R. Site1:EcoRI
R. Site2:NotI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer: