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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:NCI_CGAP_Lu19
Organism:Homo sapiens
UniGene Library ID:1661
Tissue Description:Lung, squamous cell carcinoma, poorly differentiated, 4 pooled tumors including primary and metastatic lesions
Library Keywords:lung, poorly differentiated, bulk, adult, normalized, CGAP, EST, Soares normalized, phagemid, oligo-dT primed, lung squamous cell carcinoma

Clones and Sequences

#Clones Generated to Date:10752
#Sequences Generated to Date:6901

Library Preparation Details

Description:1st strand cDNA was prepared from pooled lung tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went througb one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:
R. Site2:
Lab Host:DH10B (phage-resistant)
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Christopher A. Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D.
Library Producer:Soares Laboratory