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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares_fetal_lung_NbHL19W
Organism:Homo sapiens
UniGene Library ID:415
Tissue Description:Fetal lung, 19 weeks
Library Keywords:fetus, lung, normal, bulk, normalized, CGAP, EST, Soares normalized, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:23040
#Sequences Generated to Date:25339

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. This library was constructed from the same fetus as the fetal heart library, Soares fetal heart NbHH19W.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (ampicillin resistant)
Vector:pT7T3D (Pharmacia) with a modified polylinker
Vector Type:Phagemid
Tissue Supplier:
Library Producer:Soares Laboratory