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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares_pregnant_uterus_NbHPU
Organism:Homo sapiens
UniGene Library ID:474
Tissue Description:Pregnant uterus
Library Keywords:uterus, normal, bulk, pregnant, adult, normalized, CGAP, EST, Soares normalized, female, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:26880
#Sequences Generated to Date:30948

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid
Tissue Supplier:
Library Producer:Soares Laboratory