Skip Navigation
NCI banner National Cancer Institute U.S. National Institutes of Health National Cancer Institute
Tissues
  • Tools
  • SNPs by tissue
  • CGAP Data
Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Brn25
Organism:Homo sapiens
UniGene Library ID:1331
Tissue Description:Brain, anaplastic oligodendroglioma, pooled from 3 individuals
Library Keywords:brain, oligodendroglioma, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, male, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:29952
#Sequences Generated to Date:23439

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATAGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Myrna R. Rosenfeld M.D., Ph.D., David N. Louis, M.D.
Library Producer:Soares Laboratory