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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Brn23
Organism:Homo sapiens
UniGene Library ID:1318
Tissue Description:Brain, glioblastoma multiforme, pooled from 3 individuals
Library Keywords:brain, glioblastoma multiforme, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, directionally cloned, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:40704
#Sequences Generated to Date:23747

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATATCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier: Myrna R. Rosenfeld M.D., Ph.D., David N. Louis, M.D.
Library Producer:Soares Laboratory