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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Soares_NhHMPu_S1
Organism:Homo sapiens
UniGene Library ID:589
Tissue Description:Melanocyte, fetal heart and pregnant uterus
Library Keywords:fetus, skin, uterus, heart, normal, melanocyte, bulk, subtracted, CGAP, EST, Soares subtracted, mixed tissue, mixed developmental stages, phagemid

Clones and Sequences

#Clones Generated to Date:46848
#Sequences Generated to Date:44293

Library Preparation Details

Description:Equal amounts of plasmid DNA from three normalized libraries (melanocyte 2NbHM, pregnant uterus NbHPU, and fetal heart NbHH19W) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 260232-265223, 340488-345479, and 484488-489479.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer:Soares Laboratory