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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares_total_fetus_Nb2HF8_9w
Organism:Homo sapiens
UniGene Library ID:843
Tissue Description:Fetus, total
Library Keywords:normal, bulk, normalized, CGAP, EST, Soares normalized, embryonic tissue, first trimester, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:33024
#Sequences Generated to Date:27768

Library Preparation Details

Description:1st strand cDNA was prepared from mRNA obtained from pooled 8-9 week (total) fetus material with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer:Soares Laboratory