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Cancer Genome Characterization Initiative

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Library Info Page

Library ID

Library Name:Soares_fetal_liver_spleen_1NFLS_S1
Organism:Homo sapiens
UniGene Library ID:452
Tissue Description:Fetal liver and spleen
Library Keywords:fetus, spleen, liver, normal, bulk, normalized, subtracted, CGAP, EST, Soares subtracted, mixed tissue, male, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:36096
#Sequences Generated to Date:30930

Library Preparation Details

Description:This is a subtracted version of the original Soares fetal liver spleen 1NFLS library. 1st strand cDNA was primed with a Pac I - oligo(dT) primer [5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Pac I and cloned into the Pac I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization. Library constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Pac I
R. Site2:Eco RI
Lab Host:DH10B (ampicillin resistant)
Vector:pT7T3D (Pharmacia) with a modified polylinker
Vector Type:phagemid
Tissue Supplier:
Library Producer:Soares Laboratory