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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Soares_multiple_sclerosis_2NbHMSP
Organism:Homo sapiens
UniGene Library ID:390
Tissue Description:Multiple sclerosis lesions
Library Keywords:brain, multiple sclerosis, bulk, adult, normalized, CGAP, EST, Soares normalized, male, size fractionated

Clones and Sequences

#Clones Generated to Date:11520
#Sequences Generated to Date:15042

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from 4 multiple sclerosis lesions from one patient was kindly provided by Dr. Kevin G. Becker (NINDS/NIH).
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (ampicillin resistant)
Vector:pT7T3D (Pharmacia) with a modified polylinker V_TYPE: phagemid
Vector Type:
Tissue Supplier:Kevin G. Becker, M.D.
Library Producer:Soares Laboratory