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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:NCI_CGAP_Brn41
Organism:Homo sapiens
UniGene Library ID:5381
Tissue Description:Brain, oligodendroglioma, grade 3
Library Keywords:brain, oligodendroglioma, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:1920
#Sequences Generated to Date:883

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATCACTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.
R. Site1:NotI
R. Site2:EcoRI
Lab Host:DH10B (phage-resistant)
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:Robert Jenkins, M.D.
Library Producer:Soares Laboratory