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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Soares_NFL_T_GBC_S1
Organism:Homo sapiens
UniGene Library ID:1042
Tissue Description:Pooled normal testis, B-cell and fetal lung
Library Keywords:fetus, testis, lung, B-cell, normal, bulk, subtracted, CGAP, EST, Soares subtracted, mixed tissue, mixed developmental stages

Clones and Sequences

#Clones Generated to Date:112896
#Sequences Generated to Date:68662

Library Preparation Details

Description:Equal amounts of plasmid DNA from three normalized libraries (fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 297480-302087, 682632-687239, 726408-728711, and 729096-731399. Subtraction by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector:pT7T3D-PacI
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer:Soares Laboratory