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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


Library Info Page


Library ID

Library Name:Soares_parathyroid_tumor_NbHPA
Organism:Homo sapiens
UniGene Library ID:417
Tissue Description:Parathyroid, sporadic adenomas
Library Keywords:parathyroid, bulk, adult, normalized, CGAP, EST, Soares normalized, benign, phagemid, parathyroid tumor

Clones and Sequences

#Clones Generated to Date:27648
#Sequences Generated to Date:22987

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTTTTTTT-3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by Bento Soares and M.Fatima Bonaldo. RNA from sporadic parathyroid adenomas was kindly provided by Dr. Stephen Marx, National Institute of Diabetes and Digestive and Kidney Diseases, NIH.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (ampicillin resistant)
Vector:pT7T3D (Pharmacia) with a modified polylinker
Vector Type:phagemid
Tissue Supplier:Stephen Marx, M.D.
Library Producer:Soares Laboratory