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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Gessler Wilms tumor
Organism:Homo sapiens
UniGene Library ID:898
Tissue Description:
Library Keywords:kidney, Wilms' tumor, non-normalized, bulk, EST, unknown developmental stage, plasmid vector, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:6198

Library Preparation Details

Description:RNA was prepared from a pool of 6 anonymous Wilms' tumor RNAs. RNA was prepared by acid-phenol, followed by one round of oligo dT selection. cDNA library preparation was with the BRL/Life Tech. Superscript Plasmid system. An oligo-dT NotI primer for first strand synthesis generated gcggccgccc(t)n at the 3' end of the clones. A 5' SalI adaptor was used with sequence 5'-gtcgacccacgcgtccg-3'. Resulting cDNAs were size selected (average size 2 kb), NotI digested, and ligated into NotI/SalI-cut pSPORT1. Library was constructed by Dr. Manfred Gessler.
R. Site1:SalI
R. Site2:NotI
Lab Host:DH10B
Vector Type:plasmid (ampicillin resistant)
Tissue Supplier:
Library Producer: