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Cancer Genome Characterization Initiative

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Library Info Page


Library ID

Library Name:Soares_pineal_gland_N3HPG
Organism:Homo sapiens
UniGene Library ID:342
Tissue Description:Pineal gland
Library Keywords:normal, non-normalized, bulk, pineal gland, CGAP, EST, Soares non-normalized, unknown developmental stage, phagemid, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:13440
#Sequences Generated to Date:6509

Library Preparation Details

Description:1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B (ampicillin resistant)
Vector:pT7T3D (Pharmacia) with a modified polylinker
Vector Type:phagemid
Tissue Supplier:
Library Producer:Soares Laboratory