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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:Soares_testis_NHT
Organism:Homo sapiens
UniGene Library ID:628
Tissue Description:Testis tissue
Library Keywords:testis, normal, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, male, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:56832
#Sequences Generated to Date:51198

Library Preparation Details

Description:1st strand cDNA was prepared from mRNA obtained from Clontech Laboratories, Inc., and primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization to Cot5, and was constructed by Bento Soares and M. Fatima Bonaldo.
R. Site1:Not I
R. Site2:Eco RI
Lab Host:DH10B
Vector Type:phagemid (ampicillin resistant)
Tissue Supplier:
Library Producer:Soares Laboratory