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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

Library Info Page

Library ID

Library Name:HM3
Organism:Homo sapiens
UniGene Library ID:852
Tissue Description:
Library Keywords:skeletal muscle, normal, non-normalized, bulk, adult, EST, female, size fractionated, plasmid vector, directionally cloned, oligo-dT primed

Clones and Sequences

#Clones Generated to Date:
#Sequences Generated to Date:16350

Library Preparation Details

Description:The library was constructed by G. Lanfranchi. This library is not subtracted nor normalized. The first strand cDNA was primed with a biotinylated oligo-dT-NotI primer (5'-biotin-AACCCGGCTCGAGCGGCCGCTTTTTTTTTTTTTTTTTT-3'). The ds cDNA was sonicated and size-selected in the range 350-550 bp. The 3' specific fragments were selected by streptavidin coated magnetic beads, ligated to non-palindromic BstXI adapters, NotI digested and directionally cloned into BstXI-NotI cut pcDNAII vector.
R. Site1:BstXI
R. Site2:NotI
Lab Host:
Vector:pcDNAII (Invitrogen)
Vector Type:Plasmid
Tissue Supplier:
Library Producer: