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Cancer Genome Characterization Initiative

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SAGE Library Info

Library ID

New SAGE Library Name:SAGE_Prostate_carcinoma_CL_LNCaP-C
Old SAGE Library Name:
Organism:Homo sapiens
Library Keywords:prostate, carcinoma, cell line, short SAGE, SAGE, non-normalized

Tag Info

#Total tags:46,248
#Tags excluding linkers:40,029
#Unique tags:15,561

Tissue Info

Tissue:prostate
Cell/Histology type:carcinoma
Tissue preparation:cell line
Mutations:null
Patient age:null
Sex:null
Other info:LNCaP cells were maintained in RPMI 1640 (Life Technologies, Inc., Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS, Life Technologies, Inc., Gaithersburg, MD) and experiments were performed on cells between passages 20 and 30. For the studies of androgen regulation, charcoal/dextran stripped androgen-free FBS (cFBS, Gemini Bio-Products, Inc., Calabasas, CA) was used. LNCaP cells were cultured first in RPMI 1640 with 10% cFBS for 5 days and then stimulated with an non-metabolizable androgen analog R1881 (DUPONT, Boston, MA) 10-8 M for 24 hours (for generating LNCaP-T library). LNCaP cells identically treated but without R1881 treatment served as control (for generating LNCaP-C library). Cells were harvested at indicated time and polyA+ RNA was double-selected with Fast Track kit (Invitrogene) SAGE libraries were generated according to the procedure provided by Dr. Kinzler (Johns Hopkins University School of Medicine, Baltimore, MD).

Library Preparation Info

Tagging enzyme:BsmFI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:LNCaP cells (Item Number CRL-1740, American Type Culture Collection, Rockville, MD)
Library preparer:L. L. Xu
Prepared in lab of:S. Srivastava, Uniformed Services University of the Health Sciences
References:L. L. Xu et al., Genomics 66, 257-63 (2000)
L. L. Xu et al., Int J Cancer 92, 322-8 (2001)