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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

SAGE Library Info

Library ID

New SAGE Library Name:SAGE_Cartilage_dedifferentiated_chondrosarcoma_metastasis_CL_UIGD0
Old SAGE Library Name:
Organism:Homo sapiens
Library Keywords:cartilage, dedifferentiated, chondrosarcoma, metastasis, cell line, adult, female, short sage

Tag Info

#Total tags:109,862
#Tags excluding linkers:109,862
#Unique tags:37,892

Tissue Info

Cell/Histology type:Dedifferentiated chondrosarcoma lung metastasis
Tissue preparation:cell line
Patient age:45 years old
Other info:Tissue section of lung right lower lobe, middle diaphragmatic metastatic lesion from 45 years old female with metastatic dedifferentiated chondrosarcoma were used to obtain mono-dispersed cells. The cell line was established from the cells after culturing in RPMI-1640 supplemented with 10% FBS and 0.1% gentamicin sulfate (Gemini Bioproducts). The cell line had gone through 4 doublings to yield 2 x 107 cells. The cell line was routinely screened for mycoplasma sps. Using a PCR-based ELISA detection assay (Roche). Cell manipulations were always performed on 80-90% confluent cultures for consistency. mRNA was isolated using a direct mRNA extraction kit.

Library Preparation Info

Tagging enzyme:BsmFI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:Hendrix, M. Seftor, E. Dep of Anatomy and Cell Biology, Holden Comprehensive Cancer Center (University of Iowa).
Library preparer:S. Malchenko
Prepared in lab of:B. Soares Laboratory (University of Iowa), contributed to the National Cancer Institute Genome Anatomy Project