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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

SAGE Library Info

Library ID

New SAGE Library Name:SAGE_Cartilage_chondrosarcoma_grade_2_CL_UIFU0
Old SAGE Library Name:
Organism:Homo sapiens
Library Keywords:cartilage, cell line, chondrosarcoma, grade ii, male, adult, short sage

Tag Info

#Total tags:75,019
#Tags excluding linkers:75,019
#Unique tags:26,233

Tissue Info

Cell/Histology type:chondrosarcoma grade 2.
Tissue preparation:cell line
Patient age:28 years old
Other info:Tissue section of nterior medial aspect of the pelvis from 28 years old male with grade 2 recurrent chondrosarcoma was divided, by gross analysis, into a cartilage-like sample and was used to obtain mono-dispersed cells. The cell line was established from the cells after culturing in growth medium that contains heat inactivated FBS (Life Technologies Gibco) (12%), DMEM, glucose (4.5 gm/l), HEPES buffer (25 mM) solution and gentamicin sulfate (50 ??g/ml) (Gemini Bioproducts). The cell line had gone through 4 doublings to yield 2 x 107 cells. The cell line was routinely screened for mycoplasma sps. Using a PCR-based ELISA detection assay (Roche). Cell manipulations were always performed on 80-90% confluent cultures for consistency. mRNA was isolated using a direct mRNA extraction kit.

Library Preparation Info

Tagging enzyme:BsmFI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:J. Stevens Dep of Orthopedic Surgery (University of Iowa)
Library preparer:S. Malchenko
Prepared in lab of:B. Soares Laboratory (University of Iowa), contributed to the National Cancer Institute Genome Anatomy Project