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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


SAGE Library Info

Library ID

New SAGE Library Name:SAGE_Retinal_Pigment_Epithelium_normal_B_4PeriRPE
Old SAGE Library Name:
Organism:Homo sapiens
Library Keywords:retina, retinal pigment epithelium, choroid, normal, bulk, human, male, female, adult, short sage

Tag Info

#Total tags:90,382
#Tags excluding linkers:90,199
#Unique tags:39,534

Tissue Info

Tissue:retina
Cell/Histology type:retinal pigment epithelium (RPE) and choroid
Tissue preparation:bulk
Mutations:null
Patient age:40-59 year old pool of 5 donors
Sex:male and female
Other info:RPE/choroid libraries were made from 4 mm diameter punches of macular RPE/choroid (4MacRPE) and matching 4 mm diameter punches of mid-peripheral RPE/choroid, (4PeriRPE), taken from five donor eyes. While these libraries were made from RPE/choroid, subsequent analysis has revealed significant content of retinal transcripts in addition to RPE markers.

Library Preparation Info

Tagging enzyme:BsmFI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:null
Library preparer:Jessica Ebright
Prepared in lab of:Catherine Bowes Rickman, Duke University Eye Center
References:Bowes Rickman C and Ebright JN, et al. Defining the Human Macula Transcriptome and Candidate Retinal Disease Genes Using EyeSAGE. Invest Ophthalmol Vis Sci. 2006 Jun;47(6):2305-16.