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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.


SAGE Library Info

Library ID

New SAGE Library Name:SAGE_Muscle_normal_B_young
Old SAGE Library Name:
Organism:Homo sapiens
Library Keywords:muscle, normal, non-normalized, short SAGE, bulk

Tag Info

#Total tags:54,791
#Tags excluding linkers:53,947
#Unique tags:21,067

Tissue Info

Tissue:muscle
Cell/Histology type:normal muscle biopsy of vastus lateralis
Tissue preparation:bulk
Mutations:null
Patient age:null
Sex:null
Other info:Needle muscle biopsies were obtained from the vastus lateralis muscles of 8 healthy young men (21-24 yr) under standard conditions. All were screened fro good health and normal neuromuscular function by a panel of laboratory tests and examination by neuromuscular disease specialist. None were involved in weight lifting or extreme levels of aerobic exercise. They were asked to refrain from activities more strenuous than walking for 3 days before the biopsy. They stayed at the University of Rochester GCRC the night before the biopsy, where they received a standard meal. They received a standard breakfast about 90 min before the biopsy. The tissue was removed whitin a few minutes of administration of the local anesthetic (lidocaine). It was quickly frozen in liquid nitrogen, then stored at -70 deg C until RNA extraction.

Library Preparation Info

Tagging enzyme:BsmFI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:null
Library preparer:null
Prepared in lab of:Stephen Welle, University of Rochester
References:S. Welle, K. Bhatt, C. A. Thornton, Genome Res. 9, 506-13 (1999)