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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

SAGE Library Info

Library ID

New SAGE Library Name:mSAGE_Embyonic_stem_cell_normal_GM-CSF_IL3_LPS_CL
Old SAGE Library Name:
Organism:Mus musculus
Library Keywords:embryonic stem cell, normal, cell line, differentiated, dendritic cell, short sage, gm-csf, il3, lps, pcr directed strategy, cdna template

Tag Info

#Total tags:
#Tags excluding linkers:31,670
#Unique tags:14,642

Tissue Info

Tissue:stem cell
Cell/Histology type:null
Tissue preparation:cell line
Patient age:null
Other info:Dendritic cells were generated from the CBA/Ca mouse derived embryonic stem cell line ESF 116 (Fairchild P., et al., Curr. Biol. 10:1515-8 (2000)). These were mature by treatment with bacterial derived LPS overnight (Fairchild et al, Methods in Enzymology, in press (2003)). cDNA was preamplified (SMART) before SAGE analysis. [SAGEMap name: ES derived DCs (LPS treated)]

Library Preparation Info

Tagging enzyme:BsmFI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:null
Library preparer:null
Prepared in lab of:Kathleen Nolan, Paul Fairchild, Stephen Cobbold, Herman Waldmann