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SAGE Genie
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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

SAGE Library Info

Library ID

New SAGE Library Name:mSAGE_T_cell_tolerated_skin_graft_normal_CL
Old SAGE Library Name:
Organism:Mus musculus
Library Keywords:t-cell, normal, cell line, pcr directed strategy, cdna template, short sage

Tag Info

#Total tags:
#Tags excluding linkers:22,096
#Unique tags:7,959

Tissue Info

Tissue:white blood cells
Cell/Histology type:null
Tissue preparation:cell line
Patient age:null
Other info:A CD4+ Treg/Tr1-like H-Y + H2Ek specific line grown from the accepted male skin grafts of female A1(M) TCR transgenic mice. mRNA was derived from CD4+ T cells (>99%) 7 days from last stimulation, and contains <0.1% mast cells. cDNA was preamplified (SMART) before SAGE analysis.[SAGEMap name: Treg line SKA from skin graft]

Library Preparation Info

Tagging enzyme:BsmFI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:null
Library preparer:null
Prepared in lab of:Diana Zelenika, Elizabeth Adams, Susan Humm, Luis Graca, Sara Thompson, Steve Cobbold, Herman Waldmann