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SAGE Genie
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Cancer Genome Characterization Initiative

Visit the database of genomic characterization data for multiple tumor types.

SAGE Library Info

Library ID

New SAGE Library Name:mSAGE_T_cell_normal_anti-CD3_AP_CD4+CD25-
Old SAGE Library Name:
Organism:Mus musculus
Library Keywords:t-cell, antibody purified, normal, spleen lymphocyte, pcr directed strategy, cdna template, anti-cd3, short sage

Tag Info

#Total tags:
#Tags excluding linkers:21,279
#Unique tags:8,197

Tissue Info

Tissue:white blood cells
Cell/Histology type:null
Tissue preparation:antibody purified
Patient age:null
Other info:CD4+CD25negative spleen cells were purified from naive CBA/Ca mice by MACS sorting. T cells were activated overnight by solid phase anti-CD3. cDNA was preamplified (SMART) before SAGE analysis. [SAGEMap name: CD4+CD25neg activated]

Library Preparation Info

Tagging enzyme:BsmFI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:null
Library preparer:null
Prepared in lab of:Luis Graca, Sara Thompson, Chun-Yen Lin, Elizabeth Adams, Steve Cobbold, Herman Waldmann